Genetic diversity and comparative study of genomic DNA extraction protocols in Tamarix L. species

The genus Tamarix consists of about 54 species that mainly grow in saline areas deserts and semi-deserts. This is chemically characterized by the presence tannins, flavonoids, anthocyanins essential oils which interfere with extraction pure genomic DNA. Thus it necessary to optimize protocols minimize influence these compounds lowest level. present study compares efficiency five different approaches extract total DNA species, showing significant differences extracted contents quality,by using Kit (DNP TM Kit), CTAB method Murray Thompson, Sahu et al., Nalini al. Bi for from species. Our results showed between methods. quantity quality were checked spectrophotometer, Nano-Drop agarose gel electrophoresis analysis. Finally, a PCR-based was also applied verify amplification two molecular markers (ITS ISSR).. In study, genetic diversity 96 individuals 8 populations studied 10 ISSR markerswhile nrDNA ITS samples used. provided best (207 ng/μL) terms ofDNA. proposed this could be effective plants same polysaccharides, proteins polyphenols components. advantage simple fast as does not involve time consuming steps such incubation at higher temperatures, do requires expensive chemicals proteinase K, liquid nitrogen. ,. success obtaining high-quality has been demonstrated group reliability discussed.